Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract only
Publication Acceptance Date: 11/9/2010
Publication Date: 1/15/2011
Citation: Zanella, R., Casas, E., Snowder, G.D., Wenz, J., Neibergs, J.S., Moore, D., Neibergs, H.L. 2011. BTA2 and BTA26 are linked with bovine respiratory disease and associated with persistent infection of bovine viral diarrhea virus [abstract]. Plant and Animal Genome XIX Conference. Poster No. P522. Interpretive Summary:
Technical Abstract: Bovine viral diarrhea virus is a pathogen associated with bovine respiratory disease (BRD). BRD causes 28% of all cattle deaths and an annual U.S. loss over $692 million. The objective of this study was to refine the linkage of BRD and association of bovine viral diarrhea-persistent infection (BVD-PI) on BTA2 and BTA26. One hundred seventy-four and 210 SNPs were chosen to cover a region of 15,262 and 14,021 kb on BTA2 and BTA26, respectively. Thirty SNPs on BTA2 and 29 SNPs on BTA26 were removed due to >10% failure or low MAF (<1%). Fine mapping for BRD was conducted with 2 crossbred families. Seventy-two BRD-affected and 146 BRD-unaffected half-sibs were analyzed with QTL Express (Seaton et al. 2002) using a one QTL model with 5,000 permutations to compute chromosome-wide F statistics. Association analysis was conducted with PLINK (Purcell et al. 2007) on 63 BVD-PI calves, 50 of their dams and 60 BVD-PI-unaffected calves from the same herds. Three animals (2 BVD-PI calves and 1 dam) were removed as <90% of their genotypes were called. Associations were determined, after Bonferroni correction, for BVD-PI calves compared to unaffected calves and for dams of BVD-PI calves and unaffected calves. BRD was linked (P<0.05) to BTA2 at 139,075,760 bp (Btau_4.0) and on BTA26 at 33,695,934 bp. An association (P<0.05) with BVD-PI calves was identified on BTA2 (137,385,203 bp) and BTA26 (132,168,986 bp) and with BVD-PI dams on BTA26 (42,551,644 bp and 46,608,559 bp) suggesting that loci linked with BRD are different than those associated with BVD-PI.