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Title: Chemical and Toxicological Fate of Fumonisin B1 during Extrusion Processing of Corn Grits

item JACKSON, LAUREN - Us Food & Drug Administration (FDA)
item JABLONSKI, JOSEPH - Us Food & Drug Administration (FDA)
item Voss, Kenneth
item HANNA, MILFORD - University Of Nebraska
item BIANCHINI, ANDREIA - University Of Nebraska
item BULLERMAN, LLOYD - University Of Nebraska
item RYU, DOJIN - Texas Woman'S University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2010
Publication Date: 6/7/2010
Citation: Jackson, L.S., Jablonski, J., Voss, K.A., Hanna, M.A., Bianchini, A., Bullerman, L.B., Ryu, D. 2010. Chemical and Toxicological Fate of Fumonisin B1 during Extrusion Processing of Corn Grits. Fungi in Food and Beverages:New Research on Spoilage, Mycotoxins and Prevention. Jun 7-9,2010. Freisling, Germany.

Interpretive Summary:

Technical Abstract: Two batches of flaking corn grits were prepared by growing Fusarium verticillioides to contain low and high levels of fumonisin B1 (FB1), Batch-1 at 9.7 ppm and Batch-2 at 50 ppm FB1 as determined by HPLC. These two batches were extruded (Batch-1E; Batch-2E) or extruded with 10% w/w glucose supplementation (Batch-1EG; Batch-2EG) using a twin-screw extruder. FB1 concentrations after extrusion were: Batch-1E = 2.7 ppm; Batch-1EG = 0.6 ppm; Batch-2E = 18 ppm; and Batch-2EG = 5.7 ppm. These values corresponded to FB1 reductions of 72% (Batch-1E), 94% (Batch-1EG), 64% (Batch-2E) and 89% (Batch-2EG). Levels of N-(1-Deoxy-D-fructos-1-yl) FB1 ranged 1.9-2.4 % and 38-46% of total FB1 species when corn grits were extruded without glucose and with added glucose, respectively. Hydrolyzed fumonisin B1 was a minor species representing <15% of the total FB1 species present. In toxicological evaluation with rats, relative kidney weights were decreased in groups fed the Batch-1 or Batch-2 diets while apoptotic tubule lesions were found from animals fed the Batch-1, Batch-1E, Batch-2, Batch-2E, or Batch-2EG diets. The severity of the kidney lesions was in correspondence with the FB1 concentration in the diet. Overall, glucose supplementation enhanced FB1 reductions and in vivo toxicity of the extruded corn grits.