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Title: Obligate biotrophy features unraveled by the genomic analysis of the rust fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici

item Duplessis, Sebastien
item Cuomo, Christina
item Lin, Yao-cheng
item Aerts, Andrea
item Tisserant, Emilie
item Veneault-fourrey, Claire
item Joly, David
item Hacquard, Stephane
item Amselem, Joelle
item Cantarel, Brandi
item Chiu, Readman
item Couthinho, Pedro
item Feau, Nicolas
item Field, Mathew
item Frey, Pascal
item Gelhaye, Eric
item Goldberg, Jonathan
item Grabherr, Manfred
item Kodira, Chinnappa
item Kohler, Annegret
item Kues, Ursula
item Lindquist, Erika
item Lucas, Susan
item Mauceli, Evan
item Morin, Emmanuelle
item Murat, Claude
item Pearson, Matthew
item Quesneville, Hadi
item Rouhier, Nicolas
item Sakthikumar, Sharadha
item Schmutz, Jeremy
item Selles, Benjamin
item Shapiro, Harris
item Tangay, Philippe
item Tuskan, Gerald
item Van De Peer, Yves
item Henrissat, Bernard
item Rouze, Pierre
item Schein, Jacquie
item Dodds, Peter
item Zhong, Shaobin
item Hamelin, Richard
item Birren, Bruce
item Grigoriev, Igor
item Szabo, Les
item Martin, Francis

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/31/2011
Publication Date: 5/31/2011
Citation: Duplessis, S., Cuomo, C.A., Lin, Y., Aerts, A., Tisserant, E., Veneault-Fourrey, C., Joly, D., Hacquard, S., Amselem, J., Cantarel, B., Chiu, R., Couthinho, P., Feau, N., Field, M., Frey, P., Gelhaye, E., Goldberg, J., Grabherr, M., Kodira, C., Kohler, A., Kues, U., Lindquist, E., Lucas, S., Mauceli, E., Morin, E., Murat, C., Pearson, M., Quesneville, H., Rouhier, N., Sakthikumar, S., Schmutz, J., Selles, B., Shapiro, H., Tangay, P., Tuskan, G.A., Van De Peer, Y., Henrissat, B., Rouze, P., Schein, J., Dodds, P.N., Zhong, S., Hamelin, R.C., Birren, B.W., Grigoriev, I.V., Szabo, L.J., Martin, F. 2011. Obligate biotrophy features unraveled by the genomic analysis of the rust fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici. Phytopathology. 108:9166-9171.

Interpretive Summary: Rust fungi (Pucciniales, Basidiomycota), is a diverse group of plant pathogens composed of more than 120 genera and 6,000 species and are one of the most economically important groups of pathogens of native and cultivated plants. They are obligate biotrophic parasites that have evolved specialized feeding structures structures, formed within host tissue, to efficiently acquire nutrients and suppress host defense responses. The genomes of two evolutionarly divergent rust fungi, Puccinia graminis (wheat stem rust pathogen) and Melampsora larici-populina (leaf rust pathogen of poplar) were sequenced. Genomic features specific to the obligate biotrophic life-style were identified. This represents the first genome wide characterization of any rust fungi and provides critical information that will facilitate a better understanding of the complex interactions between these pathogens and their hosts. This knowledge will enable scientist to develop new approaches to control these economically important plant pathogens.

Technical Abstract: Rust fungi are some of the most devastating pathogens of crop plants. They are obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart from their hosts. Their lifestyle has slowed the dissection of molecular mechanisms underlying host invasion and avoidance of plant innate immunity. We sequenced the 101–mega–base pair genome of Melampsora larici-populina, the causal agent of poplar leaf rust, and the 89–mega–base pair genome of Puccinia graminis f. sp tritici, the causal agent of wheat and barley stem rust. We then compared the ~16,850 predicted proteins of M. larici-populina to the 18,240 predicted proteins of P. graminis f. sp tritici. Genomic features related to their obligate biotrophic life-style include expanded lineage-specific gene families, a large repertoire of effector-like small secreted proteins (SSPs), impaired nitrogen and sulfur assimilation pathways, and expanded families of amino-acid, oligopeptide and hexose membrane transporters. The dramatic upregulation of transcripts coding for SSPs, secreted hydrolytic enzymes, and transporters in planta suggests that they play a role in the host infection and nutrient acquisition. Some of these genomic hallmarks are mirrored in the genomes of other microbial eukaryotes that have independently evolved to infect plants, indicating convergent adaptation to a biotrophic existence inside plant cells.