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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Insect Behavior and Biocontrol Research » Research » Publications at this Location » Publication #260692
Title: Species specific ITS-1 DNA Sequences for the identification of Orius insidiosus, Orius pumilio and Orius tristicolor.

Author
item Shirk, Paul
item Furlong, Richard
item Shapiro, Jeffrey

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/29/2009
Publication Date: 12/21/2009
Citation: Shirk, P.D., Furlong, R.B., Shapiro, J.P. 2009. Species specific ITS-1 DNA Sequences for the identification of Orius insidiosus, Orius pumilio and Orius tristicolor.. National Center for Biotechnology Information (NCBI).Public Database. Acces. No. GU214726-GU214728.

Interpretive Summary: Methods are required that can rapidly and accurately distinguish between related North American species of the Orius genus that have similar morphology. DNA barcoding has been proposed as a molecular means of identifying organisms. To facilitate development of this technology, species specific DNA sequences representing the internal transcribed spacer 1 (ITS-1) from the18S ribosomal RNA gene of Orius insidiosus, Orius pumilio and Orius tristicolor were identified and the DNA sequences submitted to the GenBank database (National Center for Biotechnology Information). These were given the GenBank Accession numbers GU214726 for the ITS-1 of Orius insidiosus, GU214727 for the ITS-1 of Orius tristicolor and GU214728 for the ITS-1 of Orius pumilio.

Technical Abstract: Methods are required that can rapidly and accurately distinguish between related North American species of the Orius genus that have similar morphology. DNA barcoding has been proposed as a molecular means of identifying organisms. To facilitate development of this technology, species specific DNA sequences representing the internal transcribed spacer 1 (ITS-1) from the18S ribosomal RNA gene of Orius insidiosus, Orius pumilio and Orius tristicolor were identified and the DNA sequences submitted to the GenBank database (National Center for Biotechnology Information). These were given the GenBank Accession numbers GU214726 for the ITS-1 of Orius insidiosus, GU214727 for the ITS-1 of Orius tristicolor and GU214728 for the ITS-1 of Orius pumilio.