Submitted to: Virology Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/21/2010
Publication Date: 11/21/2010
Citation: Huang, L.V., Zhai, S.L, Cheung, A.K., Zhang, H.B., Long, J.X., Yuan, S.S. 2010. Detection of a novel porcine parvovirus, PPV4, in Chinese swine herds. Virology Journal. 7(1):333. Interpretive Summary: In 2009, researchers at the National Animal disease Center, Ames, IA in collaboration with researchers at Washington University, St. Louis, MO identified a novel porcine parvovirus, designated PPV4, among the sick animals of the 2005 USA porcine circovirus-associated disease (PCVAD) outbreak. The current study showed that PPV4 was detected among diseased swine in China. The co-infection detection results revealed that all of the PPV4-positive samples were co-infected with porcine TT virus (PTTV) or with porcine circovirus type 2 (PCV2). Thus, this work provides a frame work to investigate the inter-relationship between PCV2, PPV4, PTTV and their pathogenesis potentials. The information obtained will advance our understanding of the biology of several swine viruses and aid the research of scientists in industry, universities and government agencies.
Technical Abstract: To determine whether the recently reported novel porcine parvovirus type 4 (PPV4) is prevalent in China, a set of PPV4 specific primers were designed and used for the molecular survey of PPV4 among clinical samples. The results indicated a positive detection for PPV4 in Chinese swine herds of 1.84% (13/705), with no PPV4 detectable in samples taken prior to 2009. To further identify the PPV4 strains in China, seven nearly full-length genomic sequences of PPV4 were determined. The lengths of those genomes range from 5400 to 5644 bp and contained three major predicted open reading frames that were similar in sequence to parvovirus. The Chinese strains shared significant genetic identity (92.2–97.6%) with two published American strains of PPV4. Phylogenetic analyses showed that PPV4 was not closely related to any other virus sequences available in the GenBank database, while the genome structure and predicted protein sequences were similar to those of the parvovirus family.