|MACCREE, M - University Of California|
|SUDARSHANA, P - Monsanto Corporation|
|PARKER, S - US Department Of Agriculture (USDA)|
|Wechter, William - Pat|
|PRESTING, G - University Of Hawaii|
Submitted to: Journal of General Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/16/2012
Publication Date: 2/15/2012
Citation: Yakabe, L.E., Maccree, M.M., Sudarshana, P., Mcclean, A.E., Parker, S.R., Wechter, W.P., Presting, G., Kluepfel, D.A. 2012. Novel primers for detection of genetically diverse virulent Agrobacterium tumefaciens bv1 strains. Journal of General Plant Pathology. DOI: 10.1007/s10327-012-0364-z.
Technical Abstract: Novel primers were developed to amplify a 243 bp fragment of an intergenic region between gene5 and tms2 on the T-DNA of Agrobacterium tumefaciens. These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence and did not generate extraneous bands, facilitating robust real-time PCR detection.