Location: Plant Science ResearchTitle: Common bean-Rhizobium symbiosis: functional genomics of legume response to abiotic stresses Author
Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 6/15/2010
Publication Date: 6/30/2010
Citation: Hernandez, G., Ramirez, M., Valdes-Lopez, O., Aparicio-Fabre, R., Yang, S.H., Naya, L., Mendoza, A.B., Nova-Franco, B., Leija, A., Fuentes, S.I., Arellano, J., Girard, L., Sanchez, F., Reyes, J.L., Vance, C.P. 2010. Common bean-Rhizobium symbiosis: functional genomics of legume response to abiotic stresses. Proceedings of the XIII Meeting of the Spanish Society of Nitrogen Fixation and II Portuguese-Spanish Congress on Nitrogen Fixation, June 15-18, 2010, Zaragoza, Spain. Available: www.ugr.es/~sefin/actas%20congresos/ActasIICLEFN2010.pdf. Interpretive Summary:
Technical Abstract: Common bean (Phaseolus vulgaris) is the world's most important grain legume for direct human consumption and a main source of proteins in Latin America and Africa. Environmental factors such as nutrient deficiency, soil acidity, and metal toxicity are important constraints for bean symbiotic nitrogen fixation (SNF) and crop production. Our research is aimed at the identification of genes, gene networks, and signaling pathways that are relevant for the response/adaptation to abiotic stresses of SNF common bean plants. We have developed a platform for bean functional genomics that includes: EST sequencing, transcriptomics, transcription factor genes and microRNA (miRNA) expression profiles, and reverse genetics in bean composite plants. Phosphorus (P) deficiency is widespread in regions where common bean is produced and is perhaps the factor that most limits SNF. We have analyzed the global gene expression and metabolic responses of nodules from common bean plants inoculated with Rhizobium tropici grown under P-deficient and P-sufficient conditions. Recently, we have used a miRNA-macroarray hybridization approach to identify miRNAs expressed in nodules of common bean grown under full-nutrient or abiotic stress conditions. Our data provide the foundation to evaluate the individual roles of miRNAs in common bean.