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Title: Optimization of the genomic DNA extraction method of silverleaf nightshade/ (Solanum elaeagnifolium /Cav.), an invasive plant in the cultivated areas within the Mediterranean region

Author
item RIPOLL, JULIE - University Of Montpellier
item BON, MARIE-CLAUDE - European Biological Control Laboratory (EBCL)
item Jones, Walker

Submitted to: Biotechnologie, Agronomie, Société et Envrionnement
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/30/2010
Publication Date: 12/1/2010
Citation: Ripoll, J., Bon, M., Jones, W.A. 2010. Optimalisation de l’extraction d’ADN génomique de la Morelle Jaune, (Solanum elaeagnifolium Cav.), une invasive des milieux cultivés en Méditerranée. Biotechnologie, Agronomie, Société et Envrionnement.

Interpretive Summary: Working with the North American invasive weed in the Mediterranean region, silverleaf nightshade, a new technique was developed for extraction of DNA. The new method yields good quality DNA that can be used for, among other things, amplification and detection of microsatellites.

Technical Abstract: The geographical origin of an invasive in the cultivated area within the Mediterranean region, silverleaf nightshade, Solanum elaeagnifolium Cav, (Solanaceae) should be identified through the analysis of genetic similarities between native and introduced populations using microsatellite markers. Beforehand, an effective and less costly method for extracting genomic DNA from dehydrated and polysaccharide–rich leaves was investigated. The original Doyle’s protocol based on the use of a cationic detergent, CTAB, was successfully optimized in order to extract high quality DNA (A260/280 ratio of 1.8; A260/230 ratio of 1.7 to 2). The DNA yields obtained with this new protocol were higher than those obtained with the commercial kit, although quality of the DNA extracted was comparable. The improvement of the protocol was further proven by the amplification of several microsatellite loci developed in eggplant, S. melongena L.