|YIN, C - Washington State University|
|HULBERT, S - Washington State University|
|SCHROEDER, K - Washington State University|
|MAVRODI, O - Washington State University|
|MAVRODI, D - Washington State University|
|DHINGRA, A - Washington State University|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/2010
Publication Date: 6/20/2010
Citation: Yin, C., Hulbert, S.H., Schroeder, K.L., Mavrodi, O., Mavrodi, D., Dhingra, A., Paulitz, T.C. 2010. Comparison of bacterial communities from inside and outside of Rhizoctonia bare patches in wheat. Phytopathology.
Technical Abstract: Rhizoctonia solani AG-8 causes distinct patches of stunted wheat in the field. Bacterial communities from bulk soil and rhizospheres of wheat were analyzed with pyrosequencing. Replicated samples were taken from inside and outside of patches; and from patches that had recovered the previous 1–2 years. Pyrosequencing was performed on amplified products from primers designed to the V3 hyper-variable region of bacterial 16S rDNA. Between 2070 and 5746 sequences of >150 bp were obtained from each sample, and these were assembled into 345 OTUs, with 151 identified to the genus level. Community diversity was higher and abundance was lower in the bulk soil compared to the rhizosphere soil. In the bulk soil, abundance was highest inside the patches. Chitinophaga and Acidobacteria GP 3 were the most abundant genera, followed by Enterobacteriaceae, Pseudomonas, Pedobacter, Variovorax, Sphingomonas, Solirubrobacter, Nitriliruptor and TM7. Flavobacterium and a single OTU in the family Enterobacteriaceae and the order Sphingobacteriales were more frequent in the rhizospheres of plants inside the patch compared to outside or recovered patches, and this was confirmed by real-time quantitative PCR. Dyella and Acidobacteria GP 7 were more frequent in recovered patches. These results show that the rhizosphere community on wheat plants in diseased patches is distinct from healthy plants, and this technique may be useful for finding organisms associated with disease suppression.