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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #256836

Title: Identification and characterization of Fusarium mexicanum causing mango malformation disease in México

Author
item RODRIQUEZ-ALVARADO, GERARDO - UNIVERSIDAD MICHOACANA DE SAN NICOLAS DE HIDALGO
item FERNANDEZ-PAVIA, SYLVIA - UNIVERSIDAD MICHOACANA DE SAN NICOLAS DE HIDALGO
item OTERO-COLINA, GABRIEL - COLEGIO DE POSTGRADUADOS
item AOKI, TAKAYKI - NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES (NIAS)
item PLOETZ, RANDY - UNIVERSITY OF FLORIDA
item O DONNELL, KERRY
item MAYMON, MARCEL - AGRICULTURAL RESEARCH ORGANIZATION OF ISRAEL
item FREEMAN, STANLEY - AGRICULTURAL RESEARCH ORGANIZATION OF ISRAEL

Submitted to: Acta Horticulture Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/31/2011
Publication Date: 5/31/2013
Citation: Rodriquez-Alvarado, G., Fernandez-Pavia, S., Otero-Colina, G., Aoki, T., Ploetz, R.C., O Donnell, K., Maymon, M., Freeman, S. 2013. Identification and characterization of Fusarium mexicanum causing mango malformation disease in México. IX International Mango Symposium. 992:377-384.

Interpretive Summary:

Technical Abstract: Herein we summarize the results of fusaria we discovered associated with mango malformation disease (MMD) in México (Otero-Colina et al., 2010). From 2002 to 2009, 142 strains were isolated from symptomatic mango inflorescences and vegetative tissues from various cultivars in eight geographically diverse Mexican states. Initially, isolates were screened for genetic diversity using arbitrarily primed-PCR and RAPD DNA markers. At least seven genetically diverse MMD-associated genotypes were detected, one of which predominated (92 of 141). Maximum parsimony analyses of a five-locus dataset comprising 5.8 kb of aligned DNA sequence data indicated that at least three phylogenetically distinct fusaria within the American clade of the Gibberella fujikuroi species complex (GFSC) may be associated with MMD. These included F. sterilihyphosum and two undescribed species. These analyses also revealed that one of the undescribed species within the American clade of the GFSC, which we formally described as Fusarium mexicanum, appeared to be the most common MMD-associated species in México. PCR primers for the specific detection of the MMD pathogens F. sterilihyphosum and F. mangiferae failed to detect F. mexicanum. This novel, genealogically exclusive species was also distinguished morphologically. In addition, Koch’s postulates were completed for this species via a pathogenicity experiment which demonstrated that it could induce malformation on nucellar seedlings of cultivar Ataulfo.