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ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Publications at this Location » Publication #255469

Title: Nutrient Acquisition and Generation of Reactive Oxygen Species Via CREA, AREA, and NOXa are Important in Pathogenicity in Mycosphaerella Graminicola

Author
item Choi, Yoon-e
item Goodwin, Stephen - Steve

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2010
Publication Date: 8/7/2010
Citation: Choi, Y., Goodwin, S.B. 2010. Nutrient Acquisition and Generation of Reactive Oxygen Species Via CREA, AREA, and NOXa are Important in Pathogenicity in Mycosphaerella Graminicola. Phytopathology.

Interpretive Summary:

Technical Abstract: Mycosphaerella graminicola is an important wheat pathogen causing significant economic loss. M. graminicola is a hemibiotroph, indicating that a biotrophic stage with nutrient uptake and a necrotrophic stage associated with a possible toxin or reactive oxygen species (ROS) are important to pathogenicity. To better understand the pathogenic mechanisms of M. graminicola, we employed over-expression strategies; selected target genes for over-expression were CREA, AREA, and NOXa, which might function as regulators in nutrient acquisition and ROS generation. Increased expressions of CREA, AREA, and NOXa were confirmed via q-RT PCR and subsequently used for pathogenicity testing. Among them, the NOXa over-expression strain, NO2, resulted in significantly increased pathogenicity. Moreover, instead of the usual filamentous growth, we observed a significant predominance of yeast-like growth in NO2, which is correlated with ROS production. Our data indicate that ROS generation via NOXa is important to pathogenicity as well as development in M. graminicola.