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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #254905

Title: Aphid Resistance: From Fine Mapping to Cultivar Development

Author
item Diers, Brian - University Of Illinois
item Kim, Ki-seung - University Of Illinois
item Hudson, Matthew - University Of Illinois
item Hill, Curt - University Of Illinois
item Hartman, Glen
item Wang, Jianping - University Of Illinois

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/10/2010
Publication Date: 8/10/2010
Citation: Diers, B., Kim, K., Hudson, M., Hill, C., Hartman, G.L., Wang, J. 2010. Aphid Resistance: From Fine Mapping to Cultivar Development [abstract]. To be submitted to 13th Biennial Molecular and Cellular Biology of the Soybean Conference 2010. Durham, NC, August 8-11, 2010. CD ROM.

Interpretive Summary:

Technical Abstract: Soybean aphid [Aphis glycines Matsumura (Hemiptera: Aphididae)] was first identified as a soybean [Glycine max (L.) Merr.] pest in North America in 2000. Since that time, infestations have reached economic thresholds annually resulting in widespread spraying of the Midwest soybean crop with insecticides. After the 2000 discovery, screening of the soybean germplasm collection for resistance resulted in the identification of accessions with antibiosis and antixenosis resistance. From these resistant accessions, two genes that confer antibiosis resistance were mapped and named Rag1 and Rag2. TaqMan SNP marker assays have been developed for these two genes and they have been successfully used in marker-assisted selection. Recent fine mapping efforts have resulted in the mapping of Rag1 to a 115 kb interval and Rag2 to a 54 kb interval. The Williams 82 sequence for the interval Rag1 maps contains two predicted nucleotide binding leucine-rich repeat (NBS-LRR) candidate resistance genes and the Rag2 interval contains one predicted NBS-LRR candidate gene. Because Williams 82 carries neither Rag1 nor Rag2, the intervals these genes map need to be cloned and sequenced from genotypes carrying the genes to identify gene candidates from the resistance sources.