Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/21/2010
Publication Date: 8/22/2010
Citation: Xu, D., Klesius, P.H., Shoemaker, C.A. 2010. Efficacy of Ichthyophthirius vaccines in channel catfish against white spot disease. In: American Chemical Society National Meeting and Exposition 2010. August 22-26, 2010. Boston, Massachusetts. p. 237.
Technical Abstract: Ichthyophthirius (Ich) is a protozoan that causes white spot disease in many cultured fish and lead to severe losses in aquaculture. Two trials were conducted to determine the efficacy and serum antibody response of different formulation of Ich vaccines in channel catfish. In trial I, catfish were immunized with: 1) 1% formalin-inactivated trophonts, 2) 3% formalin-inactivated trophonts, 3) freeze-thawed trophonts, 4) live theronts (positive control) and 5) 5% bovine serum albumin (BSA) as negative control. In trial II, catfish were immunized with sonicated trophonts at doses of 1) 5 trophonts/g of fish, 2) 10 trophonts/g of fish, 3) 20 trophonts/g of fish, and 4) 5% BSA as the control. The results showed that doses of sonicated Ich trophonts vaccine, but not the formulation, affect fish antibody responses and survival. Immunization with the live Ich theront and the sonicated Ich trophont vaccines stimulated serum antibody responses and protection in channel catfish against Ich.