Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/14/2010
Publication Date: N/A
Technical Abstract: An automated and rapid method for detection of staphylococcal enterotoxins (SE) is needed. A sandwich assay was developed using a surface plasmon resonance (SPR) biosensor for detection of staphylococcal enterotoxin A (SEA) at subpicomolar concentration. Assay conditions were optimized for capturing SEA from samples by the immobilized anti-SEA sensor followed by probing the SEA molecules by the injection of the second anti-SEA. Analysis of SEA in spiked chicken extracts resulted in r = 0.986 (+/-0.02 SD) for the captured SEA and 0.994 (+/-0.006 SD) for anti-SEA binding. SEA was detected from the binding response units (RU) of the second anti-SEA using the B/ Bo ratio where B was RU of samples and Bo was RU of zero control. SEA was detected in spiked chicken extracts at 1 ng/mL with the use of CM5 sensor chip. With use of CM3 sensor chip, SEA was detected at 0.5 ng/mL in spiked chicken tissues. These results show higher sensitivity than those reported in literature using the SPR sandwich assay for SE toxins.