Location: Sunflower and Plant Biology ResearchTitle: Development and Characterization of Two New Triticum aestivum-Daspyrum villosum Robertsonian Translocation Lines T1DS.1V#3L and T1DL.1V#3S and Their Effect on Grain Quality) Author
Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/18/2010
Publication Date: 5/6/2010
Publication URL: http://naldc.nal.usda.gov/catalog/45880
Citation: Zhao, W., Qi, L., Gao, X., Zhang, G., Dong, J., Chen, Q., Friebe, B., Gill, B.S. 2010. Development and characterization of two new Triticum aestivum-Daspyrum villosum Robertsonian translocation lines T1DS.1V#3L and T1DL.1V#3S and their effect on grain quality. Euphytica. 175:343-350. Interpretive Summary: Wheat is one of the major sources of protein for the nutrition of humans. Dasypyrum villosum (L.) Candargy is a wild relative of bread wheat with one set of chromosomes, compared to bread wheat, which has three sets. Previous studies showed that chromosome 1V of D. villosum has genes that encode for seed storage proteins that may be used to enhance the grain quality of bread wheat. As a first step in genetic transfer, the present study was initiated to exchange wheat chromosome 1D with chromosome 1V of D. villosam, and to analyze the effect on grain quality. A wheat stock containing 20 pairs of wheat chromosomes and one 1D chromosome was crossed with a wheat stock containing 21 pairs of wheat chromosomes plus one pair of 1V#3 chromosomes. The progeny containing 20 pairs of wheat chromosomes plus one 1D chromosome and one 1V#3 chromosome was selected and self pollinated. Two DNA markers for the short arm of chromosome 1V#3S of D. villosum and two DNA markers for the long arm of chromosome 1V#3L of D. villosum were developed to screen a large number of progeny to identify plants that had only the 1V#3S or 1V#3L arms. Two plants with exchange of chromosome arm 1V#3S to the wheat chromosome arm 1DL and four plants with exchange of chromosome arm 1V#3L to the wheat chromosome arm 1DS were identified from the F3 population. Our results showed that the 1V#3S arm increases gluten strength and enhances wheat quality, but the 1V#3L arm decreases gluten strength and does not enhance wheat quality.
Technical Abstract: Dasypyrum villosum (L.) Candargy is a diploid, wild relative of bread wheat Triticum aestivum L. Previous studies showed that D. villosum chromosome 1V has genes that encode for seed storage proteins that may be used to enhance the grain quality of bread wheat. As a first step in genetic transfer, the present study was initiated to develop compensating Robertsonian translocations involving wheat chromosome 1D and D. villosum chromosome 1V and to analyze their effects on grain quality. A monosomic 1D stock was crossed with the disomic addition stock DA1V#3 and the double monosomic plants (20" + 1D' +1V#3') were self pollinated. Two co-dominant STS markers (BE499250 and BE591682) polymorphic for the short arm of 1V#3S and two dominant STS markers (BE518358 and BE585781) polymorphic for the long arm of 1V#3L were developed to screen a large number of progeny to identify plants that had only the 1V#3S or 1V#3L arms. Five compensating Robertsonian heterozygous translocations, two (plants #56 and #83) for the short arm (T1DL.1V#3S) and three (plants #7, #123, and #208) for the long arm (T1DS.1V#3L) were identified from 282 F2 plants and confirmed by genomic in situ hybridization and C-banding analyses. Two homozygous translocations T1DL.1V#3S (plants #14 and #39) were identified from 52 F3 plants derived from F2 plant #83. Four homozygous translocations T1DS.1V#3L (plants #3, #22, #29, and #30) were identified from 68 F3 plants derived from F2 plant #208. The homozygous translocation T1DL.1V#3S had a significantly higher (37.4 ml) and T1DS.1V#3L had significantly lower (10 ml) Zeleny sedimentation value compared to that of Chinese Spring wheat (30.7 ml). Our results showed that 1V#3S increases gluten strength and enhances wheat quality, but 1V#3L decreases gluten strength and does not enhance wheat quality.