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United States Department of Agriculture

Agricultural Research Service

Title: Sperm viability - Determination of sperm viability using fluorescence microscopy)

Author
item Donoghue, Ann - Annie
item Blanco, Juan

Submitted to: Book Chapter
Publication Type: Book / chapter
Publication Acceptance Date: 3/1/2010
Publication Date: 3/16/2010
Citation: Donoghue, A.M., Blanco, J. 2010. Sperm viability - Determination of sperm viability using fluorescence microscopy. In: Bakst, M.R., Long, J. A. editors. Techniques for Semen Evaluation, Semen Storage and Fertility. 2nd edition. St. Paul, MN: Midwest Poultry Federation. p. 39-41.

Interpretive Summary:

Technical Abstract: To determine the percentage of viable sperm in a semen sample using stains that differentiates viable (live) sperm from nonviable (dead) sperm. Viable sperm are detected by SYBR-14, which stains the sperm nuclei green. Nonviable sperm are detected by propidium iodide (PI), which stains the sperm red. The propidium iodide is not incorporated into live sperm with intact membranes.

Last Modified: 8/24/2016
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