|Liao, Huihong - Guangxi Academy Of Agricultural Sciences|
|Bai, Yang - Guangxi Academy Of Agricultural Sciences|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/10/2010
Publication Date: 6/1/2010
Citation: Lin, H., Liao, H., Bai, Y., Civerolo, E.L. 2010. A new molecular diagnostic tool for quantitatively detecting and genotyping “Candidatus Liberibacter species”. Phytopathology. 100:S72.
Technical Abstract: A new molecular diagnostic method was developed for quantitative detection of “Candidatus Liberibacter” species associated with citrus Huanglongbing (“Ca. Liberibacter asiaticus”, “Ca. Liberibacter africanus” and “Ca. Liberibacter americanus”) and potato zebra chip disorder (“Ca. Liberibacter solanaceraum”). This detection system employs a pair of universal primers designed in sequences conserved among the four Liberibacter species. Polymorphism due to deletions, insertions and nucleotide substitutions in the amplicons among the four Liberibacter species can be distinguished based on high resolution melting curve analyses. In contrast to multiplex PCR or multiple sets of primers or primers/probe commonly used for detection of different Liberibacter species, this diagnostic system, using only one pair of primers, greatly simplifies detection and eliminates competition between primer/primer and/or primer/probe combinations that may occur in multiplex systems. The assay is robust and cost-effective for reliable detection, quantification and identification of Liberibacter species in plants and insect vectors. This new diagnostic system is suitable for high throughput screening for regulatory and epidemiological studies in locations where multiple Liberibacter species may be present.