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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Publications at this Location » Publication #250865

Title: Interactive effects of active Saccharomyces cerevisiae and its cell wall material on intestinal microbial ecology during the receiving period of stressed beef cattle

Author
item Collier, Chad
item Carroll, Jeffery - Jeff Carroll
item Corley, Jimmie - Lesaffre Yeast
item Estefan, Antonio - Lesaffre Yeast
item Finck, Derek - Texas Tech University
item Johnson, Brad - Texas Tech University

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 7/11/2010
Publication Date: 10/11/2010
Citation: Collier, C.T., Carroll, J.A., Corley, J., Estefan, A., Finck, D., Johnson, B. 2010. Interactive effects of active Saccharomyces cerevisiae and its cell wall material on intestinal microbial ecology during the receiving period of stressed beef cattle [abstract]. 2010 American Society of Animal Science Meeting, July 11-15, 2010, Denver, CO. Journal of Animal Science. 88(E-Supplement 2):W17.

Interpretive Summary:

Technical Abstract: The effects of active Saccharomyces cerevisiae (SC) addition and/or S. cerevisiae cell wall (CW) material on intestinal microbial profiles were evaluated in receiving beef cattle (203 +/- 1.45 kg) during the first 56 d on feed. Cattle were assigned to 1 of 4 treatment groups; with SC (n=5); with CW (n=4); with SC and CW (n=6) and without (control; n=5). After 38 d on feed, the cattle were fitted with indwelling jugular catheters. Escherichia coli-derived lipopolysacharide (0.25 ug/kg BW) was administered via jugular catheter on d 39 and the cattle monitored for 24 h. Upon completion of the trial, fecal samples were collected from the metal housing of indwelling rectal probes inserted ~27 cm to minimize carcass and environmental sample contamination. Isolated nucleic acids were PCR amplified using prokaryotic 16S-V3-specific primers. Denaturant gradient gel electrophoresis (DGGE) was used to separate the resultant amplicons based on their unique 16S-V3 base-pair configurations. The PCR-DGGE band numbers (bacterial species) were counted, and the banding patterns analyzed by calculating Sorenson’s pairwise similarity coefficients (C(S)), an index measuring common bacterial species between samples. Total band numbers present (23.5 +/- 1.3 vs. 29.6 +/- 0.8, respectively) and band intensity were greatest (P < 0.05) in SC-treated cattle when compared to CW-treated cattle suggesting a more species- and numerically-dense microbial profile. Intra-treatment band number variations were greatest (P < 0.05) in control (+/- 3.6 bands) and SC/CW-treated (+/- 5.5 bands) cattle when compared to SC- (+/- 1.6 bands) and CW-treated (+/- 2.5 bands) cattle. Intra-treatment C(S) values were high, ranging between SC/CW (68.3 +/- 1.2) and SC (75.6 +/- 0.8). Inter-treatment C(S) comparison values were lower (P < 0.05) than intra-group values. Cumulatively, the C(S) values indicate homogenous microbial profiles within treatments that were unique from each other treatment. These results suggest that the performance and immunological modulation of SC- and CW-treated cattle are partially the result from modifying the intestinal microbial ecology. As the dynamic nature of the hindgut microbiota typically reestablishes a pre-treatment profile after perturbation or treatment, the sustained altered microbial profile observed here indicates that supplementation during the receiving period may select for a more beneficial microbial profile persisting past supplementation removal.