Location: National Clonal Germplasm RepositoryTitle: In Vitro Cold-Storage Duration of Sour Cherry (Prunus cerasus L) Shoots is Affected by Carbon Source and Nitrogen Concentration) Author
Submitted to: Acta Horticulturae
Publication Type: Abstract Only
Publication Acceptance Date: 2/15/2010
Publication Date: 9/5/2010
Citation: Kovalchuk, I., Nasibulinal, A., Reed, B.M. 2010. In Vitro Cold-Storage Duration of Sour Cherry (Prunus cerasus L) Shoots is Affected by Carbon Source and Nitrogen Concentration. Acta Horticulturae. 548. Interpretive Summary: Cold storage of fruit crop plant as tissue cultures is useful for preservation of heritage or commercial cultivars. Shoot cultures of three sour cherry cultivars were cold stored at refrigerator temperatures in either tissue culture bags or glass jars. Combinations of sugars were tested in growth medium. Nitrogen concentrations were also tested. Shoot cultures of the three cherry cultivars could be stored for 21/2 years at refrigerator temperatures and remained in excellent condition in some treatments. Sucrose was the best sugar for the three genotypes and allowed storage for up to three years. Shoots stored on mannitol survived for only 6 to 12 months while the combination of mannitol and sucrose extended storage to 2 1/2 years for two cultivars. Fifty-seven sour cherry types were cold stored in tissue culture bags on sucrose growth medium and remained in good condition for one to 2 1/2 years. The 68 types of sour cherries are now stored in tissue culture bags with sucrose.
Technical Abstract: In vitro cold storage of fruit crop germplasm is useful for preservation of heritage or commercial cultivars. Shoot cultures of sour cherry (Prunus cerasus L.) cultivars Dolgozdannaya, Moya Radost and Zukovskaya, were cold stored at 4°C in either five-section tissue culture bags or in 150 ml glass jars. Carbon sources 3% sucrose, 2% or 3% mannitol, or 2% sucrose + 2% mannitol were tested in Murashige and Skoog (MS) medium with or without plant growth regulators (PGRs). Nitrate nitrogen at 100%, 50% or 25% of the normal MS concentration was also tested. Shoot cultures of the three cherry cultivars could be stored for over 30 months at 4 °C and remained in excellent condition in some treatments. There was significant variation in the storage duration with interactions of the cultivar, treatment, and container. Sucrose was the best carbon source for all three genotypes and allowed storage for up to 36 months. Shoots stored on 2% or 3% mannitol survived for only 6 to 12 months while the combination of 2% mannitol and 2% sucrose extended storage to 30 months for two of the three genotypes. The addition of abscisic acid to 3% sucrose MS medium significantly decreased storage length. Fifty-seven accessions of sour cherry germplasm were stored in tissue culture bags on 3% sucrose MS medium without PGRs and remained in good condition for 13 to 30 months. The 68 accessions of the in vitro Prunus cerasus germplasm collection are now stored in tissue culture bags with MS medium, 0.5 mg/l BAP, 0.1 mg/l IBA, and 3% sucrose.