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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #250010

Title: Genome Sequence of Mycobacterium avium subspecies paratuberculosis Sheep Isolate 397 Reveals Striking Differences Among a Genetically Homogeneous Subspecies

item Bannantine, John
item Alt, David
item Bayles, Darrell
item SREEVATSAN, SRINAND - University Of Minnesota
item KAPUR, VIVEK - Pennsylvania State University
item TALAAT, ADEL - University Of Wisconsin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/23/2010
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The genome of Mycobacterium avium subspecies paratuberculosis is remarkably homogeneous among the bovine, human and wildlife isolates. However previous work in our labs using genomic DNA hybridizations with the bovine K-10 DNA microarray has revealed substantial differences in sheep isolates. A draft genome sequence was determined for the U.S. sheep isolate 397 using Roche/454 sequencing and optical mapping technologies. At this writing, there are less than 80 gaps remaining. Collectively, the sheep 397 isolate appears to have acquired approximately 4 large sequence regions that are shared with M. avium subsp hominisuis strain 104 and lost 3 regions that are present in the bovine K-10 strain. This sequence may help explain the phenotypic differences observed with sheep strains of M. avium subspecies paratuberculosis and provide a more complete understanding of the genomic diversity of this economically significant veterinary pathogen.