Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/6/2010
Publication Date: 6/17/2010
Citation: Vinje, M.A., Willis, D.K., Duke, S.H., Henson, C.A. 2010. Differential RNA Expression of Bmy1 During Late Seed Development in Wild and Cultivated Barley and the Association With ß-Amylase Activity [abstract]. American Society of Brewing Chemists, June 15-18, 2010, Providence, Rhode Island. Poster No. 54.
Technical Abstract: Four genotypes carrying different ß-amylase 1 (Bmy1) intron III alleles (Bmy1.a, Bmy1.b, Bmy1.c, and Bmy1.d) were analyzed for differences in Bmy1 DNA sequence, Bmy1 RNA expression, ß-amylase activity and protein, and total protein during late seed development. Wild barleys Ashqelon (Bmy1.c) and PI 296897 (Bmy1.d) had 2.5- to 3-fold higher Bmy1 RNA expression than cultivars Legacy (Bmy1.a) and Harrington (Bmy1.b). Large insertion/deletions in the Bmy1 promoter and third intron do not appear to affect the amount of Bmy1 mRNA. Approximately 503 bp upstream of the Bmy1 gene are highly conserved among cultivated and wild barleys and may contain transcription factor binding sites necessary for Bmy1 expression. Bmy1 expression patterns and putative transcription factor binding sites in the promoter indicate Bmy1 may be under the control of seed storage protein transcription factors. ß-Amylase activities per mg protein were not significantly different at maturity between all genotypes whereas Ashqelon and PI 296897 have significantly higher activities per g fresh weight than Legacy and Harrington because wild barleys had more total protein. These data indicate the wild barley genotypes produce more total proteins during seed development causing an increase in Bmy1 expression and Bmy1 protein, thus higher ß-amylase activity per mg fresh weight.