Location: Forage and Livestock Production ResearchTitle: The effects of single nucleotide polymorphisms (SNPs) of calpastatin (CAST) gene on meat tenderness of yak.) Author
Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2010
Publication Date: 8/4/2010
Citation: Wang, J.H., Wu, J.P., Wan, H.L., Yang, L., Wu, X.J., Brown, M.A., Zhang, L.P. 2010. The effects of single nucleotide polymorphisms (SNPs) of calpastatin (CAST) gene on meat tenderness of yak [abstract]. American Society of Animal Science Annual Meeting, July 11-15, 2010, Denver, CO. Available on-line: http://adsa.asas.org/meetings/2010/toc.asp Interpretive Summary: Abstract only.
Technical Abstract: The association of single nucleotide polymorphisms (SNPs) of calpastatin (CAST) gene with shear force of 2.54 cm steaks from M. longissimus dorsi from Gannan yaks (Bos grunniens, n=181) was studied. Yaks were harvested at 2, 3, and 4 yr of age (n=51, 59, and 71, respectively), and samples of each yak carcass were aged at 4°C for 0, 1, 3, 7, 14 or 21d. Shear force for each sample was determined at each of the six postmortem aging-day using Warner-Bratzler shear force methodology. Fragments of the yak CAST gene including exon 3 were amplified and subjected to Single Strand Conformation Polymorphism analysis. Two linkage SNPs were found within exon 3 of the CAST gene: G40455A and G40463A, and there were two genotypes of GG/AA and GA/GA demonstrated respectively. The genotypic frequency of GG/AA was 72.93% compared to 27.07% for GA/GA. Statistical analysis was done using mixed model procedures. Interaction of genotype x age at harvest x aging days was demonstrated (P < 0.05) for shear force. In 2-yr-old yak, GG/AA had lesser shear force than GA/GA at the aging times of 1 and 7 days (1.15 and 0.57 kg, respectively; P < 0.05); 4-yr-old yak GG/AA had lesser shear force than GA/GA at aging times of 7 and 14 days (0.64 and 0.49 kg, respectively; P < 0.05). Genotype had little effect (P > 0.05) on shear force in 2-yr- and 3-yr-old yak averaged over aging time. In 4-yr-old yak, GG/AA had 0.40 kg lesser shear force than GA/GA (P < 0.05) averaged over aging time. Therefore, it was concluded that the CAST SNPs affect (P < 0.10) shear force of M. longissimus dorsi depending on age of yak and aging time of the meat. Further study should focus on the ontogeny of CAST gene mRNA expression.