Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: GENOMIC REGULATION OF SEASONAL INFERTILITY IN SWINE

Location: Location not imported yet.

Title: Gene expression in hypothalamus, liver and adipose tissues and feed intake response to melanocortin-4 receptor (MC4R) agonist in pigs expressing (MC4R) mutations)

Author
item Barb, Claude
item Hausman, Gary
item Romdhane, Rekaya
item Lents, Clay
item Lkhavadorj, Sender
item Qu, Long
item Cai, Weiguo
item Couture, Oliver
item Anderson, Lloyd
item Dekkers, Jack
item Tuggle, Christopher

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/20/2010
Publication Date: 3/5/2010
Citation: Barb, C.R., Hausman, G.J., Romdhane, R., Lents, C.A., Lkhavadorj, S., Qu, L., Cai, W., Couture, O.P., Anderson, L.L., Dekkers, J.C., Tuggle, C.K. 2010. Gene expression in hypothalamus, liver and adipose tissues and feed intake response to melanocortin-4 receptor (MC4R) agonist in pigs expressing (MC4R) mutations [abstract]. Meeting Abstract. p. 9.

Interpretive Summary: none.

Technical Abstract: Transcriptional profiling was used to identify genes and pathways that responded to intracerebroventricular (ICV) injection of melanocortin-4 receptor (MC4R) agonist, NDP-MSH, in pigs homozygous for the missense mutation in the MC4R, D298 allele (n = 12), N298 allele (n = 12) or heterozygous (n = 12). Feed intake (FI) was measured at 12 and 24 hr after treatment. All pigs were sacrificed at 24 hr after treament and hypothalamus, liver and backfat tissue was collected. NDP-MSH suppressed (P = 0.004) FI at 12 and 24 hr in all animals after treatment. In response to NDP-MSH, 278 genes in hypothalamus (P= 0.001), 249 genes in liver (P = 0.001) and 5066 genes in fat (P = 0.015) were differentially expressed. Pathway analysis of NDP-MSH-induced differentially expressed genes indicated that genes involved in cell communication, nucleotide metabolism, and signal transduction were prominently down-regulated in the hypothalamus. In both liver and adipose tissue, energy-intensive biosynthetic and catabolic processes were down-regulated in response to NDP-MSH. This included genes encoding for biosynthetic pathways such as steroid and lipid biosynthesis, fatty acid synthesis, and amino acid synthesis. Genes involved in direct energy-generating processes, such as oxidative phosphorylation, electron transport, ATP synthesis were up-regulated, whereas TCA associated genes, were prominently down-regulated in NDP-MSH treated pigs. Our data also indicate a metabolic switch toward energy conservation since genes involved in energy-intensive biosynthetic and catabolic processes were down-regulated in NDP-MSH treated pigs.

Last Modified: 8/24/2016
Footer Content Back to Top of Page