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Title: Identification and mapping of nucleotide binding site-leucine rich repeat resistance gene analogs in bermudagrass

item Harris-Shultz, Karen
item SCHWARTZ, BRIAN - University Of Georgia
item PATERSON, ANDREW - University Of Georgia
item BRADY, JEFF - Texas A&M Agrilife

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/28/2009
Publication Date: 1/9/2010
Citation: Harris, K.R., Schwartz, B.M., Paterson, A.H., Brady, J.A. 2010. Identification and mapping of nucleotide binding site-leucine rich repeat resistance gene analogs in bermudagrass. Plant and Animal Genome XVIII Conference, January 9-13, 2010, San Diego, CA.

Interpretive Summary: not required

Technical Abstract: Bermudagrasses, Cynodon (L.) Rich., are widely adapted to a range of environments with varying biotic stresses. Therefore, thirty-one bermudagrass disease resistance analogs (BRGA) were cloned and sequenced from diploid, triploid, and hexaploid bermudagrass using degenerate primers to target the nucleotide binding site (NBS) of the NBS-LRR resistance gene family. Alignment of deduced amino acids revealed that the conserved motifs of the NBS were present and all sequences had non- Drosophila Toll and mammalian interleukin-1 receptor (TIR) motifs. Using a neighbor-joining algorithm, a dendrogram was created and eight BRGA groups were identified. Four BRGA markers and fifteen bermudagrass expressed sequence tags (ESTs) with homology to resistance genes or resistance gene analogs were placed on a F1 bermudagrass map. BRGA and EST markers clustered on T89 linkage groups. In addition, two primers made from BRGA groups and ESTs with homology to NBS-LRR resistance genes amplify NBS-LRR homologs in zoysiagrass. This gives evidence of conservation of NBS-LRR resistance genes among the Chloridoideae subfamily. These BRGA and EST markers may be useful in marker-assisted selection for the improvement of disease resistance in bermudagrass.