Submitted to: Subtropical Technology Conference Proceedings
Publication Type: Abstract only
Publication Acceptance Date: 10/18/2009
Publication Date: 10/22/2009
Citation: Manthey, J. 2009. Fast centrifugal partition chromatography as a preparative chromatographic technique for citrus natural products. Subtropical Technology Conference Proceedings. 60:8. Interpretive Summary:
Technical Abstract: Fast centrifugal partition chromatography (FCPC) is a preparative-scale separations methodology based on the principles of counter current chromatography. Separations by FCPC are typically achieved with higher recoveries and with lower solvent use compared to conventional column chromatography. HSCPC, run with a Kromaton A200, was used to separate groups of compounds in mixtures of polymethoxylated flavones obtained from orange oil. Biphasic separations with n-hexane/ethyl acetate/methanol/water (1/0.8/1/1, v/v/v/v) solvent mixtures, using either the organic phase as the stationary phase in the descending mode, or with the aqueous phase as the stationary phase in the ascending mode allowed for efficient separations of the polymethoxylated flavones within 2 h chromatographic runs. No loss in resolution occurred for scale-up from 20 mg to 400 mg sample injection. Quantitative recoveries were achieved. Purification of the main PMFs is achieved by FCPC, as well as significant simplifications of the isolations of minor PMF constituents by subsequent column chromatography.