Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/10/2009
Publication Date: 6/10/2009
Citation: Hughes, S.R., Butt, T., Boiley, J., Bang, S., Gibbons, W., Bischoff, K.M. 2009. GMAX yeast background strain made from industrial tolerant Saccharomyces cerevisiae engineered to convert sucrose, starch and cellulosic sugars universally to ethanol anaerobically with concurrent coproduct expression [abstract]. Society for In vitro Biology. Abstract #106. p. 135. Interpretive Summary:
Technical Abstract: Tailored GMAX yeast background strain technology for universal ethanol production industrially. Production of the stable baseline glucose, mannose, arabinose, xylose-utilizing (GMAX) yeast will be evaluated by taking the genes identified in high-throughput screening for a plasmid-based yeast to utilize xylose and glucose anaerobically for ethanol production. The use of the xylose isomerase gene from Piromyces in combination with a modified xylulokinase gene from bacteria plus the anaerobic growth genes from S. cerevisiae allowed anaerobic growth on xylose and glucose simultaneously for cellulosic ethanol production and the gene set to be used as the basis for the background strain for other co-product gene expressions such as a lipase, antibacterial, and or an insecticidal gene set. The resulting stable transformed plasmids into any industrial yeast strains of Saccharomyces cervisiae that are already tolerant to environments in the production biorefinery are being developed for universal ethanol production from any feedstock provided. Initially a cellulosic strain will be produced with XI, XKS, and one or more of the anerobic xylose utilization genes to be discussed for use on acid or base hydrolysates for high level production of cellulosic ethanol and use as a platform for coproduct production.