|Kistler, H - Corby|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/10/2009
Publication Date: 9/11/2009
Citation: Kistler, H.C., Li-Jun, M., Jin-Rong, X. 2009. Comparative functional genomics of plant pathogenic Fusarium species. Meeting Abstract. p. 17. Interpretive Summary:
Technical Abstract: Fusarium species are among the most economically important group of plant pathogenic fungi. Comparison of the four currently available Fusarium genome sequences allows an unsurpassed and unprecedented ability to predict genes, determine synteny and define regulatory sequences for genes in phytopathogenic fungi. Since the species also differ in biological properties such as microconidium and ascospore formation, host range and disease phenotype, comparison of genomes in combination with whole genome gene expression analysis can be used to identify processes and pathways associated with phenotypes both common and unique to each species. Our long term goal is to build upon the available genomic resources to determine molecular mechanisms of plant pathogenesis and infection-related development processes and their regulation in these Fusarium species. The specific objectives of this proposal are: 1) Development of a multiple-oligonucleotide microarray for the whole-genomes of F. oxysporum, F. verticillioides, and F. graminearum; 2) Functional analysis of genes required for pathogenicity and host specificity; 3) Systematic analysis of sexual and asexual reproduction processes that are important for plant infection; and 4) Development of an integrated database for comparative genomic studies. The information gained through this study will significantly improve our knowledge of fungal pathogenesis and developmental biology in these important Fusarium species. By understanding the fundamental mechanisms by which the fungus reproduces, accumulates toxins and causes disease, we may be able to develop novel, stable, and environmentally sensible disease management practices aimed at interfering with these essential processes.