Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/26/2009
Publication Date: 8/22/2009
Citation: Nam, N., Kim, S.M., Domier, L.L., Koh, S., Moon, J., Choi, H.S., Kim, H.G., Moon, J.S., Lee, S.H. 2009. Nucleotide Sequences and Genomic Organization of a Newly Identified Member of the Carmovirus genus, Soybean Yellow Mottle Mosaic Virus (SYMMV), from Soybean. Archives of Virology. 154(10):1679-1684. Interpretive Summary: Soybean is one of the most widely grown oil-seed crops in the world. Virus infections reduce both the quantity and quality of seed harvested. Because soybean plants are susceptible to several different viruses, it is important to identify the virus(es) responsible for disease so that targeted breeding programs can be implemented to introduce genes for resistance into new soybean varieties and reduce losses. A new soybean infecting virus, Soybean yellow mottle mosaic virus (SYMMV), was identified in Korea. SYMMV initially induces bright yellow mosaic symptoms on leaves followed by stunting and reduced growth of older leaves. SYMMV was shown to be most similar to, but distinct from Cowpea mottle virus, which has been found only in Nigeria. Each year, many soybean plants are observed and collected with virus-like disease symptoms for which it has not been possible to identify an infecting virus. The discovery of SYMMV may allow scientists to assign a causal agent to some of these previously undiagnosed plants.
Technical Abstract: Soybean yellow mottle mosaic virus (SYMMV) was cloned and sequenced from infected soybean (Glycine max) in Korea. The complete monopartite single-stranded RNA genome of SYMMV consists of 4009 base pairs with six putative open reading frames and includes 5’- and 3’-untranslated regions of 39 and 229 nucleotides, respectively. The nucleotide and coat protein sequences of SYMMV share the highest sequence identity with Cowpea mottle virus. Based on these genomic organizations, the predicted amino acid sequences, and phylogenetic analyses with known carmoviruses, we report that SYMMV is a new member of the genus Carmovirus in the family Tombusviridae.