Location: Arkansas Children's Nutrition CenterTitle: Hepatic gene profiling prior to puberty reveals greater female predominant expression of lipogenic genes Author
Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 2/11/2009
Publication Date: 4/1/2009
Publication URL: http://www.fasebj.org.libproxy.uams.edu/content/vol23/1_MeetingAbstracts/aindex.shtml#A
Citation: Shankar, K., Ronis, M.J., Badger, T.M. 2009. Hepatic gene profiling prior to puberty reveals greater female predominant expression of lipogenic genes [abstract]. FASEB J. 23(1_MeetingAbstracts):343.6. Interpretive Summary:
Technical Abstract: We evaluated changes in transcriptomic profiles in livers of prepeubertal rats using Affymetrix microarrays. Female Sprague-Dawley rats were fed a phytoestrogen free, AIN-93G-based diet containing casein as the sole protein source from gestation day 4 and offspring were weaned onto the same diet. Male and female offspring were sacrificed on PND25 and hepatic gene expression assessed using the Rat 230 2.0 GeneChip microarrays. Data were analyzed using GeneSpring Gx7.3 and Ingenuity Pathway Analyses and transcripts that changed +/-1.8-fold (p <0.05) were enriched. Expression of 60 transcripts was significantly altered between genders. Among these, genes involved in lipid biosynthesis and elongation, adiponutrin (11-fold), fatty acid synthase (4-fold), ATP citrate lyase (2.9-fold), ELOVL6 (5-fold), acetyl-coA carboxylase (2-fold) and thyroid hormone responsive Spot 14 (2-fold) were increased in females compared to males. Importantly, mRNA expression of the master lipogenic factor SREBP-1 was increased 2.2-fold in female liver. In addition, genes regulating fatty acid oxidation, Cpt1a, CYP4A14 and Slc22a5 and cholesterol transporters Abcg5, Abcg8 were lower in female livers (p<0.05). These results indicate significant gender differences in SREBP-1 regulated lipogenic gene expression prior to divergence in major sex hormones at puberty.