Submitted to: Journal of Chromatography B
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/25/2010
Publication Date: 4/9/2011
Citation: Ayinampudi, S.R., Wang, Y., Avula, B., Smillie, T.J., Khan, I.A. 2011. Quantitative analysis of oxyresveratrol in different plant parts of Morus species and related Genera by HPTLC . Journal of Chromatography B. 24(2):125-129. Interpretive Summary: This publication is related to isolation, HPLC and HPTLC quantification of a marker oxyresveratrol. Oxyresveratrol is a bioactive compound related to stilbene. In this publication, we did quantitative and qualitative analysis of oxyresveratrol in different plant samples almost fifteen plant samples from same genius. This work is useful to know the quantity and availably of marker in different plant samples. This information is very useful for further proceedings.
Technical Abstract: Four aromatic compounds; oxyresveratrol (1), mulberroside A (2), cudraflavone C (3) and kuwanone J (4) were isolated from the stems of Morus rubra L. The quantitative determination of oxyresveratrol from M. rubra L., M. alba L. and related genera by high performance thin layer chromatography (HPTLC) and HPLC methods are described. The methods were validated for selectivity, extraction efficiency, sensitivity, accuracy, intra- and inter-day reproducibility studies. The extraction efficiency was in the range of 100±3.2%. The limit of detection and limit of quantification of oxyresveratrol in the plant samples was found to be 50 ng/spot, 0.3 µg/mL and 200 ng/spot, 1.0 µg/mL, respectively by HPTLC and HPLC methods. This is the first report of analytical methods developed for the quantitative analysis of oxyresveratrol from stems, stem-leaves and leaves of M. rubra L. by HPLC with PDA detection and HPTLC. The amount of oxyresveratrol was found higher in stems than in stem-leaves and was not detected in leaves. UV detection was used in both methods and was performed at 327 nm. These methods were found to be simple and sensitive with good precision and reproducibility.