|NAGL, NEVENA - University Of Novi Sad|
Submitted to: Journal of Biotechnology
Publication Type: Abstract Only
Publication Acceptance Date: 6/30/2007
Publication Date: 7/1/2007
Citation: Nagl, N., Weiland, J.J., Lewellen, R.T. 2007. Detection of DNA polymorphisms in sugarbeet bulks by SRAP and RAPD markers Meeting Abstract 136:1 S1-S772 doi:10.1016/j.biotec.2007.07.051
Technical Abstract: The random amplified polymorphic DNA (RAPD) marker system has been used in many different applications involving the detection of DNA sequence polymorphism, but most often in construction of linkage maps and in bulk segregant analysis (BSA) for identification of markers linked to genes of interest. The sequence-related amplified polymorphism (SRAP) technique is relatively a new and highly efficient PCR-based technique, based on amplification using two primers. The primers are designed to preferentially amplify exonic and intronic regions, and create polymorphisms that depend on variation of the length of introns, promoters and spacers. In this report we present results in the development of fast and inexpensive RAPD and SRAP marker protocols and discuss their potential use in identification of markers linked to gene(s) for resistance to sugar beet cyst nematode (Heterodera schachtii Schmidt) using BSA.