|Van earden, David|
Submitted to: Mycological Society of America
Publication Type: Abstract only
Publication Acceptance Date: 6/1/2009
Publication Date: 7/25/2010
Citation: Pitcher, K., Rivera, Y., Van Earden, D., Baldwin, T.T., Kretzer, A. 2010. Population genetic analyses of the ectomycorrhizal basidiomycete Suillus spraguei (synonym S. pictus). Mycological Society of America. July 25-30,2009. Snowbird, UT. Inoculum 60(3):35-36. Interpretive Summary: Abstract - no summary required.
Technical Abstract: Suillus spraguei is a specific symbiont of Pinus strobus in the eastern United States. We found that previously developed microsatellite markers for S. spraguei in Japan were unsuccessful at amplifying DNA isolated from sporocarps found in New York State, and other research suggests that they are disjunct species. Using NY specimens, we identified nine polymorphic microsatellite loci and developed primers to amplify these regions. Sporocarps were collected from a total of 7 sites in NY, PA, and MA. Based on a sporocarp map created for one site in Clymer, NY, we determined that the distance between sporocarps with the same multilocus genotype was small (<10 m) and most sporocarps were genetically unique. We determined that we could use 7 of the 9 markers to efficiently distinguish genets. Therefore all other sites were scored with at least 7 markers. At all sites, the majority of the markers were found to be in Hardy-Weinberg and linkage equilibrium. Little to no genetic differentiation was detected between sites (F/ST=0.0-0.099) and there was no correlation between genetic and geographic distance (isolation by distance). Overall, these results show that the population structure of S. spraguei is characterized by frequent sexual reproduction and gene flow.