|KAPUR, VIVEK - Pennsylvania State University|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 9/1/2009
Publication Date: 1/1/2010
Citation: Paustian, M., Bannantine, J.P., Kapur, V. 2010. Mycobacterium avium subsp. paratuberculosis Genome. In: Behr, M.A., Collins, D.M., editors. Paratuberculosis Organism, Disease, Control. Wallingford, Oxfordshire, UK: CABI. p. 73-82.
Technical Abstract: The completion of the MAP K-10 genome sequence has opened the doors to many new avenues of research. In the few years since the publication of the genome sequence, the manuscript describing the completed sequence has been cited in the scientific literature more than 85 times. The public availability of a completed genome sequence has allowed researchers to include MAP in comparative genomic studies with other mycobacteria, which in turn has facilitated the transfer of information from other fields of research. This has led to new insights into mechanisms of virulence, diagnostic targets, and the molecular evolution of MAP as a pathogen. The genome sequence has facilitated the development of DNA microarrays for use in genome-wide comparative genomic hybridizations and transcriptional analyses. The systematic cloning and expression of a large subset of MAP proteins from the annotated genome sequence has been initiated. In addition, the use of proteomic techniques such as tandem mass spectrometry has been enabled by the availability of a reference genome from which to identify the genes that encode observed peptide sequences. The anticipated completion of additional mycobacterial genome projects in the near future should further add to the utility of the MAP genome sequence.