|CHEN, SHIYAN - Cornell University - New York|
|LU, SHUN-WEN - Cornell University - New York|
|YU, HANG - Cornell University - New York|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/16/2009
Publication Date: 8/1/2009
Citation: Chen, S., Lu, S., Yu, H., Wang, X. 2009. Identification and functional analysis of a candidate parasitism gene Gr-33E05 of the potato cyst nematode Globodera rostochiensis. Meeting Abstract. Phytopathology 99:52.
Technical Abstract: The potato cyst nematode, Globodera rostochiensis, has evolved an intimate parasitic relationship with host plants by transforming selected root cells into a specialized feeding structure called syncytium that provides essential nutrients for the feeding nematode. Secreted proteins encoded by parasitism genes expressed within the nematode’s esophageal gland cells are the major molecules responsible for the formation of syncytium. We cloned a putative parasitism gene (Gr-33E05) from G. rosotchiensis and also determined its genomic structure. The genomic sequence of Gr-33E05 consists of six introns and seven exons. Interestingly, additional cloning and sequencing analysis identified two splice variants of Gr-33E05 that are revealed to be generated by alternative 5’ splice site selection in the third intron. Both Gr-33E05A and Gr-33E05B encode putative secreted proteins that differ by only a 22-amino acid (aa) segment with Gr-33E05A but not Gr-33E05B containing the 22-aa segment. Transcripts of Gr-33E05 were found to be exclusively accumulated within the dorsal gland cell of both preparasitic and parasitic stages of the nematode, suggesting a role of its encoded products in nematode parasitism. Functional analyses including analyzing the effects of Gr-33E05 overexpression in potato hairy roots and Arabidopsis and nematode infection assays on transgenic potato hairy roots expressing dsRNA targeting Gr-33E05 are underway to discover the role of Gr-33E05 in plant parasitism.