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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #240634

Title: Hidden Gems in the Mycobacterium avium subsp. paratuberculosis Genome

item Bannantine, John
item TALAAT, ADEL - University Of Wisconsin
item Paustian, Michael
item SREEVATSAN, SRINAND - University Of Minnesota

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/9/2009
Publication Date: 8/9/2009
Citation: Bannantine, J.P., Talaat, A.M., Paustian, M., Sreevatsan, S. 2009. Hidden Gems in the Mycobacterium avium subsp. paratuberculosis Genome [abstract].

Interpretive Summary:

Technical Abstract: If 4,350 genes annotated in the M. avium subsp paratuberculosis strain K-10 genome wasn’t already enough to study, more genes have recently been uncovered, hidden deep within this genome sequence. Genomic and proteomic studies, both published and unpublished, have revealed a handful of new genes missed by annotation of the K-10 genome. BLAST searches of the intergenic regions present in the annotation shows several new genes. Furthermore, an automated annotation of the K-10 genome by the Venter Institute (JCVI) has revealed an additional 188 genes. Most of the genes missed in the original annotation are located between two annotated genes (intergenic region) while a few others are either discovered as part of a misalignment or exist on the opposite DNA coding strand. A catalog of these genes will be presented with a focus on three selected genes of special interest in diagnostics and pathogenesis. One of these encodes a protein that is detected by a monoclonal antibody that uniquely binds to M. avium subsp paratuberculosis.