|Matsumoto Brower, Tracie|
Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/8/2009
Publication Date: 7/18/2009
Citation: Matsumoto, T.K., F.T.P. Zee, J.Y. Suzuki, S. Tripathi, B. Mackey, R. Hollingsworth, M. Shintaku and L. Keith. 2009. Protection and conservation of Caricaceae germplasm with PRSV resistant transgenic papaya. Plant Biology 2009. Honolulu, HI. P09021 pg. 166. Interpretive Summary:
Technical Abstract: Papaya ringspot virus (PRSV) is a devastating disease that has a detrimental impact on both commercial papaya production and Caricaceae germplasm conservation. The PRSV coat protein transgenic line 55-1 and derived progeny are resistant to PRSV and have saved the papaya industry in Hawaii. Here we present preliminary information on a method to protect susceptible Caricaceae germplasm using PRSV resistant transgenic papayas as border planting to limit aphid transmitted infection. Similar to transgenic crops throughout the world there is public concern on cross contamination of transgenic material into non-transgenic lines. As the designated germplasm repository for Caricaceae we are responsible for maintaining the genetic integrity of each accession. Therefore, we have also developed a protocol utilizing polymerase chain reaction (PCR) for the detection of adventitious presence of transgenic material in both the parental plants and the resulting seed population by testing for the 55-1 transformation event to assure a 99.9% chance of obtaining greater than 99.5% transgene free seeds. The protocol developed in this study is not typical for most seed validation techniques since there is a higher than normal rejection rate for rejecting seed lots; however, we believe this is necessary to ensure the genetic integrity of seeds stored in the repository.