|Goodman, Cynthia - Cindy|
Submitted to: Society of Invertebrate Pathology
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/2009
Publication Date: 8/19/2009
Citation: Popham, H.J., Goodman, C.L., Grasela, J.J. 2009. Protein Expression Profiles of Permissive, Semi-Permissive and Non-Permissive Cells Infected by Baculovirus [abstract]. Society of Invertebrate Pathology. p. 89.
Technical Abstract: Amassing information on the in vitro protein expression of an insect host challenged by an entomopathogenic agent, such as a baculovirus, is paramount to an enhanced understanding of how host-pathogen interactions determine the success or failure of a pathogen. In this study, 2D-gel electrophoresis and subsequent mass spectrometric analysis employing MALDI TOF/TOF were used to investigate the protein expression of three insect cell lines exposed to baculoviruses for 24 h, including (1) Heliothis virescens cells permissive to an Autographa californica multiple nucleopolyhedrovirus (AcMNPV) challenge, (2) H. virescens cells semi-permissive to Helicoverpa zea single nucleopolyherovirus (HzSNPV) infection, and (3) H. zea cells non-permissive to AcMNPV infection. We found a major shift in the pattern of protein expression between mock-treated controls and H. virescens cells exposed to AcMNPV (permissive) as well as HzSNPV (semi-permissive). Of the 24 proteins identified in the H. virescens mock-treated controls, 21 were either completely absent or present at extremely low expression levels in the AcMNPV infected cells. The three remaining proteins showed an increase in expression levels 2- to 4.3-fold greater than in mock-treated controls. Two of these proteins were identified as signal transduction proteins and one as a DNA supercoiling factor. In the semi-permissive system, changes in protein expression were also found that were suggestive of the protein expression observed in the permissive system. In contrast, the number and expression levels of identified H. zea cell proteins exposed to the non-homologous AcMNPV after 24 h showed little change from mock-infected controls. This is somewhat expected as it is based on the nature of the relationship revealed by in vitro and in vivo studies between the non-permissive H. zea cells challenged with AcMNPV.