Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 5/10/2009
Publication Date: 5/10/2009
Citation: Yeh, H., Klesius, P.H. 2009. Channel Catfish, Ictalurus punctatus, CD63 cDNA. Genbank. Accession No. FJ899742.
Technical Abstract: CD63, also known as lysosome associated membrane protein 3 (LAMP-3), is a member of tetraspanin integral membrane protein family. This protein plays many important roles in immuno-physiological functions. In this communication, we cloned, sequenced and characterized the channel catfish, CD63 transcript. The complete nucleic acid sequence of channel catfish CD63 cDNA comprised of 1,159 nucleotides, including an open reading frame, which appears to encode a putative peptide of 237 amino acid residues. By comparison with human CD63, the channel catfish CD63 peptide can be divided into domains including four transmembrane domains, three intracellular domains, and one of each small and large extracellular loops. The channel catfish CD63 transcript was detected by RT-PCR in spleen, head kidney, liver, intestine, skin and gill. This result provides important information for further elucidating CD63 in channel catish.