Location: Location not imported yet.Title: Clonal mature adipocyte production of proliferative-competent daughter cells requires lipid export prior to cell division) Author
Submitted to: International Journal of Stem Cells
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/2/2009
Publication Date: 5/20/2009
Publication URL: www.ijstemcell.com
Citation: Chen, J., Guridi, M., Fernyhough, M.E., Jiang, Z., Guan, L.L., Hausman, G.J., Dodson, M.V. 2009. Clonal mature adipocyte production of proliferative-competent daughter cells requires lipid export prior to cell division. International Journal of Stem Cells. 2:76-79. Interpretive Summary: Mature fat cells from cattle and pigs may resume cell division and add to the population of fat cell fibroblasts or form other cell types. Cultures of mature pig-derived fat cells began to reestablish their ability to divide. The fat contained within the cytoplasm was either moved to ends of the cell, or large fat droplets were physically extruded from the cell. The cell lipid droplet was handled in a different manner to that by beef-derived fat cells. This difference in fat handling and trafficking may represent a novel mechanism in fat cell resumption of proliferation.
Technical Abstract: Numerous in vitro observations have been published to show that mature adipocytes may resume proliferation and begin to populate the adipofibroblast fraction or form other cell types. In the present study, we evaluated clonal cultures of mature pig-derived adipocytes as they began to reestablish their ability to divide. The lipid contained within the cytoplasm was either moved to the apical ends of the cell, or large droplets were physically extruded from the cell. In the latter case, we ascertained that the cell lipid droplet was handled in a different manner to that by beef-derived adipocytes as described in other published studies. Pig-derived adipocytes expel large amounts of lipid directly into the medium environment prior to becoming capable of cell division, rather than retaining all lipids like the beef cells. This difference in lipid handling and trafficking may be a novel mechanism in adipocyte resumption of proliferation.