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Title: Ambiguous genetic relationships among coconut (Cocos nucifera L.) cultivars: the effects of outcrossing, sample source and size, and method of analysis.

Author
item MAURO-HERRERA, MARGARITA - Oklahoma State University
item Meerow, Alan
item PERERA, LALITH - Coconut Research Institute Of Sri Lanka
item RUSSELL, JOANNE - Scottish Crops Research Institute (SCRI)
item Schnell Ii, Raymond

Submitted to: Genetic Resources and Crop Evolution
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/22/2009
Publication Date: 7/31/2009
Citation: Mauro-Herrera, M., Meerow, A.W., Perera, L., Russell, J., Schnell Ii, R.J. 2009. Ambiguous genetic relationships among coconut (Cocos nucifera L.) cultivars: the effects of outcrossing, sample source and size, and method of analysis.. Genetic Resources and Crop Evolution. 57:203-217.

Interpretive Summary: Using 41 DNA genetic markers we show that inferred relationships of coconut varieties from germplasm collections is strongly influenced by undetected hybridization between varieties in the sampled collections, as well by the method used to analyze the data. Therefore, the currently accepted scenario of coconut domestication may be an inaccurate depiction.

Technical Abstract: A prior analysis of eight coconut cultivars with 15 microsatellite (SSR) markers drew unexpected relationships between two of the out-crossing tall cultivars evaluated: ‘Atlantic Tall’ and ‘Panama Tall’. We further investigated the relationships between these eight cultivars by increasing the number of individuals studied (particularly for ‘Atlantic Tall’ and ‘Panama Tall’), by including 28 more molecular markers, and by adding two other cultivars to our analysis. Our results show that five to ten coconut individuals do not represent a dependable sample to withdraw conclusions regarding cultivar/variety relationships, particularly when studying out-crossing genotypes. As suggested in the prior study, a high level of hybridization was observed between the ‘Atlantic Tall’ and ‘Panama Tall’ cultivars. However, at this time we were able to identify distinct groups for each one of these two cultivars. The two clustering methods used (Neighbor Joining, NJ and Unweighted Pair Group Method with Arithmetic mean, UPGMA) produced dendrograms that resolved contrasting cultivar relationships, especially for the ‘Atlantic Tall’ and ‘Panama Tall’ cultivars. We discuss the implications of our results in regard to current scenarios of coconut domestication and future considerations when assessing genetic relationships among different varieties.