Submitted to: PLoS One
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/4/2010
Publication Date: 2/3/2010
Publication URL: http://handle.nal.usda.gov/10113/40678
Citation: Metzgar, D., Myers, C., Russell, K., Faix, D., Blair, P., Brown, J., Vo, S., Swayne, D.E., Thomas, C., Stenger, D., Lin, B., Malanowski, A., Wang, Z., Blaney, K., Long, N., Schnur, J., Saad, M., Borsuk, L., Lichanska, A., Lorence, M., Weslowski, B., Schafer, K., Tibbetts, C. 2010. Single assay for simultaneous detection and differential identification of human and avian influenza virus types, subtypes, and emergent variants. PLoS One. 5(2):e8995. DOI: 10.1371/journal.pone.0008995. Interpretive Summary: Rapid and accurate detection, identification and genetic characterization are essential for effective monitoring for influenza viruses. This report describes applications of a new biotechnology method capable of simultaneous obtaining gene sequences of influenza virus and 29 other viral and bacterial pathogens that have been associated with respiratory illness. The new assay was better at detecting and subtyping influenza viruses, than other molecular methodologies. This will save time and reduce the cost for identifying respiratory pathogens.
Technical Abstract: Rapid and accurate detection, identification and genetic characterization are essential for effective surveillance and epidemiological tracking of influenza viruses. This report describes applications of a resequencing pathogen microarray (RPM) assay that is capable of simultaneous sequencing of subtypes of Type A and Type B influenza virus hemagglutinin and neuraminidase genes; selected influenza virus matrix (M), NS and PB2 genome segments; and multiple representative genes of 29 other viral and bacterial pathogens that have been associated with influenza-like illness. The RPM assay has equivalent to greater sensitivity and superior specificity for detection and subtype identification of influenza viruses, and it delivers significantly greater decision-quality information per assay than traditional serological subtyping assays or more recently adopted polymerase chain reaction (PCR)-based methodologies.