Submitted to: World Association of Veterinary Laboratory Diagnosticians
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2009
Publication Date: 6/17/2009
Citation: Susta, L., Miller, P.J., Hu, S., Liu, Z., Rue, C.A., Afonso, C.L., Brown, C.C. 2009. Pathogenesis study of selected velogenic strains of Newcastle disease virus in White Leghorn chickens [abstract]. In: Proceedings of the 14th International Symposium for the World Association of Veterinary Laboratory Diagnosticians, WAVLD 2009, June 17-20, 2009, Madrid, Spain. p. 222. Interpretive Summary:
Technical Abstract: Groups of 4-week old White Leghorn chickens were inoculated intraconjunctivally with three Newcastle disease viruses isolated from natural outbreaks (Vietnam, Australia, both velogenic; and U.S., mesogenic) and two strains rescued by reverse genetics (ZJ1 and ZJ1-GFP). The parent ZJ1, a velogen, was responsible for outbreaks in Southern China. Birds were monitored clinically and euthanized sequentially with collection of tissues for histopathological examination, immunohistochemistry for viral nucleoprotein and in situ hybridization using an anti-sense digoxigenin labeled riboprobe corresponding to the sequence of the matrix gene. Disease was severe in all velogenic strains, with acute systemic illness, high mortality rates, necrosis of lymphoid tissue, and detection of nucleoprotein in multiple tissues. Encephalitic lesions, consisting of perivascular cuffing, were most severe in birds inoculated with the Australia and U.S strains, perhaps because these birds survived the longest. A unique finding was the marked tropism of the Australia strain for myenteric plexus neurons in the intestine.