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United States Department of Agriculture

Agricultural Research Service

Title: Components of Plasma Selenium in Healthy Americans

item Jackson, Matthew
item Johnson, Luann
item Hoeg, Antonia
item Hoefig, Carolin
item Davis, Cindy
item Milner, John
item Schomburg, Lutz
item Combs, Gerald

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 1/7/2009
Publication Date: 4/27/2009
Publication URL:
Citation: Jackson, M.I., Johnson, L.K., Hoeg, A., Hoefig, C., Davis, C.D., Milner, J.A., Schomburg, L., Combs, G.F. 2009. Components of Plasma Selenium in Healthy Americans. Journal of Federation of American Societies for Experimental Biology. 23:346.2

Interpretive Summary:

Technical Abstract: In a cohort of healthy adults (106 M, 155 W) in eastern North Dakota, we assessed the effects of supplementation with L-selenomethionine (SeMet) (0, 50, 100 or 200 mcg selenium [Se]/d for 1yr) on three plasma/serum biomarkers of Se status, which at baseline were: Se, 141.5±23.7 (SD) ng/ml; selenoprotein P (SePP), 3.55 (CI 2.61, 4.51) mg/l; glutathione peroxidase (GPX3) activity, 3.64±0.54 nmoles NADPH/min/mg. We imputed the portion of plasma Se present in selenocysteine (SeCys) in SePP and GPX3 from their known SeCys contents (5.5 and 4 g-atoms/mole), the molecular weights reported for each (57 kD and 92kD), and the enzyme number reported for purified GPX3 (2.8 x 104 nmoles NADPH/min/mg). This showed that prior to intervention 39.8±6.0 % of plasma Se occurred as SeCys, with the balance, 60.2±10.3 % comprised of other forms, presumably SeMet incorporated nonspecifically into various proteins. SeMet-supplementation did not affect the size of the SeCys component, but increased that of the non-specific component in a dose-dependent manner: baseline, 87.5±25.8 mcg/la; 12 mos.: 93.5±28.0a, 133.0±37.4b, 157.6±40.9c and 223.4±51d mcg/l for subjects receiving 0, 50, 100 or 200 mcg Se/day, respectively. Because SeMet is a dominant form of Se in foods, these results indicate that consumption of Se-containing foods by non-deficient individuals affects primarily the non-specific components of plasma Se.

Last Modified: 06/26/2017
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