Submitted to: Brazilian Archives of Biology and Technology - BABT
Publication Type: Peer reviewed journal
Publication Acceptance Date: 7/1/2011
Publication Date: 10/14/2011
Citation: Viancelli, A., Kunz, A., Esteves, P.A., Bauermann, F.V., Furukawa, K., Fujii, T., Antonio, R.V., Vanotti, M.B. 2011. Bacterial biodiversity from an anaerobic up flow bioreactor with ANAMMOX activity inoculated with swine sludge. Brazilian Archives of Biology and Technology - BABT. 54:1035-1041. Interpretive Summary: The removal of the ammonium in industrial wastewaters is conventionally carried out by the combination of two biological processes, nitrification and denitrification. In the last decades, an alternative nitrogen removal process, named anaerobic ammonium oxidation (ANAMMOX) was discovered. The advantages of this process over conventional processes are the reduction of aeration demand and energy expenditure, as well as decreased production of sludge and avoidance of nitrous oxide emissions. The present study is the first Brazilian report describing the detection and molecular analysis of a bacteria with ANAMMOX activity (Candidatus Brasilis Concordiensis). The discovery of new species and its ecology can open a wide range of opportunities to improve the wastewater treatment, especially with regards to the development of new generation, low-cost livestock wastewater treatment systems in Brazil.
Technical Abstract: The present study aimed to identify organisms with ANAMMOX activity in a reactor maintained in a laboratory. Molecular methods as fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR) and cloning of 16S-rDNA genes probing for Planctomycetes were performed. Seventeen clones were obtained. The sequencing and phylogenetic analysis showed that three of them presented high identity with ANAMMOX genera, which were closely related to Planctomycete KSU-1 and Candidatus Jettenia asiatica. Among the 17 clones, 12 clustered with genus Janthinobacterium, showing to be the dominant bacteria genus in the reactor. One of the clones was identified as a member of Chlolobi genus. Low identities (91% and 93%) of 16S rRNA gene of the bacterium with those of Chlolobi suggests that it belongs to a new unknown genus of Chlolobi. The present study is the first Brazilian report describing the detection and molecular analysis of a bacteria with ANAMMOX activity (Candidatus Brasilis Concordiensis clones 1 and 2.The complete understanding of ANAMMOX activity and the organism responsible to it, can open a wide range of opportunities to improve the wastewater treatment, especially with the discovery of new species and its ecology.