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United States Department of Agriculture

Agricultural Research Service

Research Project: CONTROL OF RUSTS OF CEREAL CROPS

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Title: Identification of wheat-Thinopyrum intermedium alien disomic addition lines conferring resistance to stripe rust)

Author
item Lin, Feng
item Sun, Quan
item Xu, Shichang
item Chen, Xianming
item Zhang, Lijun
item Zhang, Chunyu
item Xu, Yufeng
item Miao, Qing
item Qu, Bo
item Li, Nan

Submitted to: Canadian Journal of Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2009
Publication Date: 5/1/2009
Publication URL: article.pubs.nrc-cnrc.gc.ca/RPAS/rpv?hm=HInit&calyLang=eng&journal=cjps&volume=89&afpf=CJPS08139.pdf
Citation: Lin, F., Sun, Q., Xu, S., Chen, X., Zhang, L., Zhang, C., Xu, Y., Miao, Q., Qu, B., Li, N. 2009. Identification of wheat-Thinopyrum intermedium alien disomic addition lines conferring resistance to stripe rust. Canadian Journal of Plant Science 89:569-574.

Interpretive Summary: Grass species Thinopyrum intermedium carries many useful traits for wheat genetic improvement. To identify genes conferring resistance to stripe rust in wheat genotype Zhong 4 (Z4) derived from a cross between common wheat and Th. intermedium, a cross was made between Z4 and a ‘Chinese Spring’ ph1b mutant (CS-ph1b). F1 and F2 plants of the cross were used to determine the mode of inheritance of resistance in Z4. Genetic analysis with stripe rust race CY31 indicated a single dominant gene confering resistance. The chromosome number in the root-tip cells of 10 F5 resistant lines (total 42 plants) was determined. Three alien addition lines (4-1-2-5-1, 5-1-2-1-1-5, 5-1-2-1-1-6) with the smallest chromosome number (2n=44) were identified. Each of the alien addition line was genetically stable. The results of genomic in-situ hybridization indicated that lines 5-1-2-1-1-5 and 5-1-2-1-1-6 were wheat-Th. intermedium addition lines with 21 pairs of wheat chromosomes and 1 pair of Th. intermedium chromosomes, while line 4-1-2-5-1 was a wheat-A. intermedium translocated-addition line with one Th. intermedium chromosome, one wheat-Th. intermedium translocated chromosome and 21 pairs of wheat chromosomes. These lines were all resistant to stripe rust. The wheat- Th. intermedium translocated-addition line (4-1-2-5-1) should be useful for wheat breeding programs for stripe rust resistance.

Technical Abstract: Thinopyrum intermedium carries many useful traits for wheat genetic improvement. To identify genes conferring resistance to stripe rust caused by Puccinia striiformis f. sp. tritici in Zhong 4 (Z4) derived from a cross between common wheat and Th. intermedium, a cross was made between Z4 and a ‘Chinese Spring’ ph1b mutant (CS-ph1b). F1 and F2 plants of the cross were used to determine the mode of inheritance of resistance in Z4. The genetic constitution of 10 F5 lines derived from the cross CS-ph1b × Z4 was analyzed by cytological observations and genomic in-situ hybridization (GISH). Of 201 F2 seedlings tested with race CY31 of P. striiformis f. sp. tritici, 147 were resistant and 54 susceptible. The segregation fit a 3 resistant:1 susceptible ratio, indicating that a single dominant gene conferred resistance to the race. Using the Shiff dyeing method, the chromosome number in the root-tip cells of 10 resistant lines (total 42 plants) was determined. Three alien addition lines (4-1-2-5-1, 5-1-2-1-1-5, 5-1-2-1-1-6) with the smallest chromosome number (2n=44) were identified. A total of 51 pollen mother cells were examined at metaphase (PMCs M) for meiotic chromosome pairing. Most chromosomes in each of the three alien addition lines were paired as a rod bivalent (48 PMCs) and only few appeared as univalents (3 PMCs), indicating that each alien addition line was genetically stable. The GISH results indicated that lines 5-1-2-1-1-5 and 5-1-2-1-1-6 were wheat-Th. intermedium addition lines with 21 pairs of wheat chromosomes and 1 pair of Th. intermedium chromosomes, while line 4-1-2-5-1 was a wheat-A. intermedium translocated-addition line with one Th. intermedium chromosome, one wheat-Th. intermedium translocated chromosome and 21 pairs of wheat chromosomes. These lines were all resistant to stripe rust. The wheat- Th. intermedium translocated-addition line (4-1-2-5-1) should be useful for wheat breeding programs for stripe rust resistance.

Last Modified: 8/24/2016
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