Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 1/13/2009
Publication Date: 12/1/2009
Citation: Ditty, J.L., Nichols, N.N., Parales, R.E. 2010. Measurement of Hydrocarbon Transport in Bacteria. In: Timmis, K.N., editor. Handbook of Hydrocarbon and Lipid Microbiology. Berlin Heidelberg: Springer-Verlag. p. 4213-4222.
Technical Abstract: Hydrocarbon uptake by bacteria has not been extensively studied, and strong evidence for active transport of hydrocarbons is lacking. The volatile nature of hydrocarbons, their hydrophobicity, and their relatively low aqueous solubilities can complicate transport assays. Here we present a detailed protocol for measuring hydrocarbon uptake in bacteria, pointing out key control experiments necessary to obtain conclusive results.