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Title: Effects of specific amino acid changes on the antigenicity of hemagglutinin molecules of avian influenza isolates from Mexico

Author
item Pfeiffer, Jennifer
item LEE, CHANG WON
item JADHAO, SAMADHAN
item Suarez, David

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2009
Publication Date: 7/11/2009
Citation: Pfeiffer, J., Lee, C., Jadhao, S., Suarez, D.L. 2009. Effects of specific amino acid changes on the antigenicity of hemagglutinin molecules of avian influenza isolates from Mexico [abstract]. American Association of Avian Pathologists Annual Meeting, July 11-15, 2009, Seattle, Washington. CD-ROM, Paper No. 7584.

Interpretive Summary:

Technical Abstract: Amino acid (aa) changes between the hemagglutinin (HA) proteins of a vaccine avian influenza virus and more recent field isolates were detected following prolonged vaccination of Mexican poultry. Using site-directed mutagenesis and reverse genetics (rg), viruses containing identical backbones but different HAs were generated. The cross-hemagglutination inhibition (HI) and virus neutralization tests were used to compare antigenic differences. A vaccine-challenge study on chickens was performed to compare differences in viral shedding between groups, as determined through real-time reverse transcriptase polymerase chain reaction (RT-PCR) on oropharyngeal swab sample ribonucleic acid (RNA). The goal was to gain knowledge for selecting vaccine seed strains.