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Research Project: MANAGEMENT OF TEMPERATE FRUIT NUT AND SPECIALTY CROP GENETIC RESOURCES

Location: National Clonal Germplasm Repository

Title: Cryopreservation of in vitro grown shoot tips

Author
item Kushnarenko, Svetlana
item Reed, Barbara
item Normah, M.

Submitted to: Plant Tissue Culture and Development
Publication Type: Book / Chapter
Publication Acceptance Date: 8/16/2010
Publication Date: 11/12/2010
Citation: Kushnarenko, S.V., Reed, B.M., Normah, M.N. 2010. Cryopreservation of in vitro grown shoot tips. Trigiano, Robert N., Gray, Dennis J., editors. Plant Tissue Culture and Development and Biotechnology. Boca Raton, Florida: Taylor and Francis (CRS Press, LLC). p. 497-506.

Interpretive Summary: This chapter in Plant Cell Culture, Development and Biotechnology describes student laboratory exercises for storage of the growing shoot tips of plants in liquid nitrogen. It includes two exercises involving step by step protocols for use with shoot tips. Vitrification (fast freezing) and encapsulation dehydration (drying) techniques are described in detail for use in undergraduate or graduate student class work.

Technical Abstract: This chapter in Plant Cell Culture, Development and Biotechnology describes student laboratory exercises for cryopreservation of the growing shoot tips of plants in liquid nitrogen. It includes two exercises involving step by step protocols for use with shoot tips. Vitrification (fast freezing) and encapsulation dehydration techniques are described in detail for use in undergraduate or graduate student class work.