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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #234645
Title: Cryopreservation of plant cells, tissues and organs

Author
item Reed, Barbara
item NORMAH, M. - UNIVERSITY MALAYSIA
item KUSHNARENKO, SVETLANA - INST PLANT BIO KAZAKHSTAN

Submitted to: Plant Cell Culture, Development and Biotechnology
Publication Type: Book / Chapter
Publication Acceptance Date: 8/15/2010
Publication Date: 11/12/2010
Citation: Reed, B.M., Normah, M.N., Kushnarenko, S.V. 2010. Cryopreservation of plant cells, tissues and organs. In: Trigiano, Robert N., Gray, Dennis J. editors. Plant Cell Culture, Development and Biotechnology. Boca Raton, Florida: Taylor and Francis (CRC Press, LLC). p. 489-496.

Interpretive Summary: This chapter in Plant Cell Culture, Development and Biotechnology describes the storage of plant cells, tissues and organs in liquid nitrogen (cryopreservation). It includes sections on basic theory, tissue culture aspects and descriptions of the main techniques used. Cryopreservation techniques are now well developed and can be used for both temperate and tropical plant materials with only modest modifications. The choice of technique is dependent on the resources of the laboratory, the type of plant material, and the characteristics of the plant.

Technical Abstract: This chapter in Plant Cell Culture, Development and Biotechnology describes cryopreservation of plant cells, tissues and organs. It includes sections on basic cryopreservation theory, tissue culture aspects of cryopreservation and descriptions of the main techniques used.Cryopreservation techniques are now well developed and can be used for both temperate and tropical plant materials with only modest modifications. The choice of technique is dependent on the resources of the laboratory, the type of plant material, and the characteristics of the plant.