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Title: Development of Fibroblast Cell Lines From the Cow Used to Sequence the Bovine Genome

Author
item Chitko-Mckown, Carol
item Macneil, Michael

Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/2/2009
Publication Date: 8/1/2010
Citation: Chitko Mckown, C.G., Macneil, M.D. 2010. Development of Fibroblast Cell Lines From the Cow Used to Sequence the Bovine Genome. Animal Genetics. 41(4):445.

Interpretive Summary: Two cell lines, designated MARC.BGCF.2 and MARC.BGCF.1-3, were developed from skin biopsies taken from the neck of the Hereford cow whose DNA was used in sequencing the bovine genome. We developed these cell lines to provide a nearly unlimited source of DNA to researchers performing genetic studies utilizing the information from the soon to be published Bovine Genome Sequence. These cell lines have been submitted to American Type Culture Collection (ATCC, Manassas, VA, USA) and will be made publicly available.

Technical Abstract: Two cell lines, designated MARC.BGCF.2 and MARC.BGCF.1-3, were initiated from skin biopsies obtained from the Hereford cow whose DNA was used in sequencing the bovine genome. These cell lines were submitted to American Type Culture Collection (ATCC, Manassas, VA, USA) and will be made publicly available. Because the amount of DNA required for genomic studies cannot be predicted, these cells will provide a relatively unlimited source of DNA for subsequent work.